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| Polylysine modification of lutein nanoliposomes andits in vitro release performance |
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| DOI： |
| KeyWord:ε-poly-L-lysine modification lutein nanoliposome release performance |
| FundProject:黑龙江省教育厅基本业务专项(粮头食尾)(LTSW201712)；黑龙江省属高校基本业务专项(植物性食品加工技术特色学科专项) (YSTSXK201806)；黑龙江省自然基金优秀青年项目(YQ2019C024)；黑龙江省博士后科研启动金资助项目(LBH-Q19193) |
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| Abstract: |
| The lutein nanoliposomes (LUT-NLP) were modified with the hydrophilic cationic polypeptide (ε-poly-L-lysine, ε-PLL) by electrostatic adsorption to develop a new nanoliposome carrier system, in order to improve the encapsulation property and release performance of fat-soluble lutein. The nanoliposomes were prepared by anti-solvent method, and the optimal modification process of lutein nanoliposomes was explored by single factor experiment and orthogonal experiment, and the structural characteristics and in vitro release performance before and after modification were also investigated. The results showed that the optimal modification conditions were obtained as follows: ε-PLL dosage 0.08%, pH 6.0 and modification time 2.0 h. Under these conditions, the encapsulation efficiency of the lutein nanoliposomes was 9536%. Dynamic light scattering (DLS) analysis demonstrated that the modified lutein nanoliposomes had an average size of (299.4 ± 8.4)nm, a lower polydispersity index (PDI) (<0.3) and an increasing membrane potential compared with the lutein nanoliposomes. Transmission electron microscopy (TEM) images showed that due to electrostatic adsorption, ε-PLL combined with the liposome surface to form a protective coating structure. In vitro release experiments revealed that ε-PLL improved the lutein release from the nanoliposomes in gastrointestinal fluid conditions. Therefore, the modification with ε-PLL could improve the structure of lutein nanoliposomes and enhance the encapsulation effect of lutein, and their release in gastric and intestinal fluid environment was also improved. |
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