|
| 杜仲籽油冻干脂质体的制备及表征 |
| Preparation and characterization of freeze-driedEucommia ulmoides seed oil liposome |
| |
| DOI： |
| 中文关键词: 杜仲籽油 冻干脂质体 冻干保护剂 储藏稳定性 |
| 英文关键词:Eucommia ulmoides seed oil freeze-dried liposome freeze-drying protectant storage stability |
| 基金项目:湖南省科技厅重点研发计划项目(2018NK2045) |
|
| Hits: 958 |
| Download times: 492 |
| 中文摘要: |
| 为增加杜仲籽油的水溶性和稳定性，提高其生物利用度，将杜仲籽油制成冻干脂质体。采用乙醇注入-超声法制备杜仲籽油脂质体悬浮液并进行冻干处理，以冻干后样品外观、再分散性及复溶后包埋率为指标优化其冻干工艺条件，分析杜仲籽油冻干脂质体的微观形态、粒径、Zeta电位与储藏稳定性，利用差示扫描量热仪（DSC）、X射线衍射仪（XRD）分析冻干保护剂在脂质体中的存在状态。结果表明：杜仲籽油冻干脂质体制备的最佳冻干工艺条件为预冻温度-50?℃、预冻时间12 h、干燥时间36 h、冻干保护剂为甘露醇、甘露醇与大豆卵磷脂质量比8∶?1。在最佳冻干条件下，杜仲籽油冻干脂质体为白色球状，复溶后包埋率为（72.52±1.95）%，粒径为（389.67±4.81）nm，PDI为0255±0.013，Zeta电位为（-22.62±1.66）mV，储藏稳定性较好。 |
| 英文摘要: |
| In order to improve the stability, solubility and bioavailability of Eucommia ulmoides seed oil, the freeze-dried Eucommia ulmoides seed oil liposome was prepared by ethanol injection-ultrasound method and freeze-drying. Using appearance, redispersibility and encapsulation efficiency after rehydration of the samples as indexes, the freeze-drying conditions were optimized. The characteristics of the liposome including microform, particle size, Zeta potential and storage stability were determined, and the differential scanning calorimetry (DSC), X-ray diffraction (XRD) were used to analyze the state of freeze-drying protectant. The results showed that the optimal freeze-drying conditions of preparing Eucommia ulmoides seed oil liposome were obtained as follows: pre-freezing temperature -50?℃, pre-freezing time 12 h, drying time 36 h, with mannitol as freeze-drying protectant, mass ratio of mannitol to soy lecithin 8∶?1. Under these conditions, the liposome was white spheroid, and the capsulation efficiency after rehydration, particle size, PDI and Zeta potential were (72.52±1.95)%, (389.67±4.81)nm, 0.255±0.013 and (-22.62±1.66)mV, respectively. The liposome obtained had good storage stability. |
| 查看全文 View/Add Comment Download reader |
| Close |
|
|
|
