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| Determination of active components content in ozonized olive oil by NMR |
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| DOI： |
| KeyWord:ozonized olive oil ozonation reaction NMR |
| FundProject:国家自然科学基金（31760016）；陕西省重大科技创新项目（2019GY-138, 2020GY-236）；农药与化学生物学教育部重点实验室开放基金(2016A02)；农业部农产品加工重点实验室开放基金(201705) |
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| Abstract: |
| The ozonized products were prepared from olive oil by ozonation reaction. The data of carbon spectrum was collected by NMR, and the characteristic peaks of 13C NMR were integrated to calculate the relative content of oxidized components in olive oil. The results showed that the carbon spectrum of olive oil had significant differences from the ozonized olive oil. The new peak appeared at δ 103.1, corresponding to the weakening of the peak height at δ 130.1, which was due to the oxidation of unsaturated double bonds in olive oil. The active component contents in five ozonized oils determined by the method were 21.46%,21.03%,21.31%,18.71% and 24.42%, respectively. The method of determining the active component content in ozonized olive oil by NMR had the advantages of simple operation, no pretreatment and reliable results. |
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