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| 核桃蛋白多肽对脂多糖诱导的小鼠急性肝损伤的保护作用 |
| Protective effect of walnut protein polypeptide on LPS-induced acute liver injury in mice |
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| DOI： |
| 中文关键词: 核桃蛋白多肽 脂多糖(LPS) 急性肝损伤 炎症因子 保护作用 |
| 英文关键词:walnut protein polypeptide lipopolysaccharide (LPS) acute liver injury inflammatory factor protective effect |
| 基金项目:云南省重大科技专项（2018ZG003） |
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| 中文摘要: |
| 构建脂多糖（LPS）诱导小鼠急性肝损伤模型，研究核桃蛋白多肽（WPP）对肝损伤的保护作用。实验小鼠随机分为5组（空白对照组，LPS模型组，LPS+WPP低、中、高剂量组），连续灌胃WPP 5周后，采用尾静脉注射LPS诱导肝损伤，检测各处理组小鼠肝脏指数、血清中谷丙转氨酶（ALT）和谷草转氨酶（AST）活性、肝组织中超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性、组织病理学变化以及血清中炎症因子白细胞介素IL-1β和IL-6水平。结果表明：与LPS模型组相比，WPP高剂量组肝脏指数显著降低（P<0.05），WPP中、高剂量组小鼠血清中ALT和AST活性极显著下降（P＜0.01），WPP低、中、高剂量组小鼠血清中IL-1β和IL-6含量极显著下降（P＜0.01），WPP中、高剂量组小鼠肝组织中SOD活性显著升高（P<0.05），WPP低、中、高剂量组小鼠肝组织中GSH-Px活性极显著升高（P＜0.01）；组织病理学观察发现，WPP低、中、高剂量组小鼠肝组织病理损伤明显减轻。WPP对LPS造成的小鼠急性肝损伤具有明显的保护作用。 |
| 英文摘要: |
| The model of acute liver injury induced by lipopolysaccharide (LPS) in mice was established to study the protective effect of walnut protein polypeptide (WPP) on liver injury. Mice were randomly divided into five groups (blank control group, LPS model group, LPS+WPP low-dose group, LPS+WPP medium-dose group and LPS+WPP high-dose group). After continuous administration of WPP for five weeks, LPS was injected into tail vein to induce liver injury. The liver index, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity in liver tissue, histopathological changes, serum IL-1β and IL-6 were detected. The results showed that compared with the LPS model group, the liver index of the mice in the WPP high-dose group was significantly reduced(P<0.05), the ALT and AST activities in the serum of mice in the WPP medium and high-dose groups decreased extremely significantly (P<001), the levels of IL-1β and IL-6 in the serum of mice in the WPP low, medium and high-dose groups decreased extremely significantly (P<001), the SOD activites in the liver tissues of mice in the WPP medium and high-dose groups increased significantly（P<0.05）, and the GSH-Px activities in the liver tissues of mice in the WPP low, medium and high-dose groups increased extremely significantly (P<0.01). Histopathological observation showed that the pathological injury of the liver tissue in the WPP low, medium and high-dose groups was significantly reduced. WPP had obvious protective effect on LPS-induced acute liver injury in mice . |
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