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| Comparison of water holding capacity, oil holding capacity, solubility and amino acid composition of flaxseed protein extracted by two methods |
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| DOI： |
| KeyWord:flaxseed protein water holding capacity oil holding capacity solubility amino acid composition double enzyme complex method alkali-solution and acid-precipitation method |
| FundProject:河北省科技厅项目（18227132D）；河北省教育厅项目（ZD2017205） |
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| Abstract: |
| With flaxseed meal as raw material, flaxseed protein was extracted by double enzyme complex method and alkali-solution and acid-precipitation method, and the water holding capacity, oil holding capacity, solubility and amino acid composition of flaxseed protein were determined and compared. The results showed that the water holding capacity of flaxseed protein extracted by alkali-solution and acid-precipitation method was better than that extracted by double enzyme complex method at pH 6-7 and 40-80 ℃. The oil holding capacity of flaxseed protein extracted by double enzyme complex method was better than that extracted by alkali-solution and acid-precipitation method at pH 4-8, but the oil holding capacity of flaxseed protein extracted by alkali-solution and acid-precipitation method was better than that extracted by double enzyme complex method at 40-100 ℃. The solubility of flaxseed protein extracted by double enzyme complex method was better than that extracted by alkali-solution and acid-precipitation at pH 2-9(except pH 3,7), 20-60 ℃ and NaCl concentration 0.3-1.5 mol/L.The flaxseed protein had rich variety of amino acids. The content of glutamic acid in flaxseed protein was the highest, and the contents of glutamic acid in flaxseed protein extracted by double enzyme complex method and alkali-solution and acid-precipitation method were 10.793% and 10615%, respectively.The total amino acid contents in flaxseed protein were similar. |
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