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| 两种方法提取的亚麻籽蛋白持水性、持油性、溶解性和氨基酸组成比较 |
| Comparison of water holding capacity, oil holding capacity, solubility and amino acid composition of flaxseed protein extracted by two methods |
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| DOI： |
| 中文关键词: 亚麻籽蛋白；持水性；持油性；溶解性；氨基酸组成 双酶复合法；碱溶酸沉法 |
| 英文关键词:flaxseed protein water holding capacity oil holding capacity solubility amino acid composition double enzyme complex method alkali-solution and acid-precipitation method |
| 基金项目:河北省科技厅项目（18227132D）；河北省教育厅项目（ZD2017205） |
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| 中文摘要: |
| 以亚麻籽饼粕为原料，分别采用双酶复合法和碱溶酸沉法提取亚麻籽蛋白，测定并比较两种亚麻籽蛋白的持水性、持油性、溶解性及氨基酸组成。结果表明：碱溶酸沉法提取的亚麻籽蛋白的持水性在pH 6~7、40~80 ℃下优于双酶复合法；双酶复合法提取的亚麻籽蛋白的持油性在pH 4~8下优于碱溶酸沉法，但碱溶酸沉法提取的亚麻籽蛋白的持油性在40~100 ℃下优于双酶复合法；双酶复合法提取的亚麻籽蛋白的溶解性在pH 2~9（pH 3和pH 7除外）、20~60 ℃、NaCl浓度 0.3~15 mol/L下优于碱溶酸沉法;亚麻籽蛋白氨基酸种类丰富，谷氨酸含量最高，双酶复合法和碱溶酸沉法提取的亚麻籽蛋白的谷氨酸含量分别为10.793%和10.615%，两种方法氨基酸总和差异较小。 |
| 英文摘要: |
| With flaxseed meal as raw material, flaxseed protein was extracted by double enzyme complex method and alkali-solution and acid-precipitation method, and the water holding capacity, oil holding capacity, solubility and amino acid composition of flaxseed protein were determined and compared. The results showed that the water holding capacity of flaxseed protein extracted by alkali-solution and acid-precipitation method was better than that extracted by double enzyme complex method at pH 6-7 and 40-80 ℃. The oil holding capacity of flaxseed protein extracted by double enzyme complex method was better than that extracted by alkali-solution and acid-precipitation method at pH 4-8, but the oil holding capacity of flaxseed protein extracted by alkali-solution and acid-precipitation method was better than that extracted by double enzyme complex method at 40-100 ℃. The solubility of flaxseed protein extracted by double enzyme complex method was better than that extracted by alkali-solution and acid-precipitation at pH 2-9(except pH 3,7), 20-60 ℃ and NaCl concentration 0.3-1.5 mol/L.The flaxseed protein had rich variety of amino acids. The content of glutamic acid in flaxseed protein was the highest, and the contents of glutamic acid in flaxseed protein extracted by double enzyme complex method and alkali-solution and acid-precipitation method were 10.793% and 10615%, respectively.The total amino acid contents in flaxseed protein were similar. |
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