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| 葵花籽蛋白脱色工艺的研究 |
| Bleaching of sunflower seed protein |
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| DOI： |
| 中文关键词: 葵花籽蛋白 脱色 限制性酶解 大孔树脂 |
| 英文关键词:sunflower seed protein bleaching limited enzymatic hydrolysis macroporous resin |
| 基金项目:国家自然科学基金地区科学基金（31860423）;内蒙古自治区科技计划项目（2020GG0064） |
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| 中文摘要: |
| 用常规方法提取的葵花籽蛋白颜色呈深灰色，严重影响其在食品中的应用，为此研究了葵花籽蛋白的脱色工艺，旨在制备浅色葵花籽蛋白。以低温脱脂葵花籽粕为原料，利用限制性酶解后再结合大孔树脂对葵花籽蛋白进行吸附处理，探讨其对葵花籽蛋白脱色效果的影响。以吸附温度、pH、吸附时间、树脂添加量为考察因素，以葵花籽蛋白的L*值为评价指标进行正交试验。结果表明：碱性蛋白酶结合AB-8型大孔树脂吸附后葵花籽蛋白的脱色效果最佳，葵花籽蛋白的最佳脱色工艺条件为pH 7.0、吸附温度20 ℃、吸附时间120 min、树脂添加量12%；在最佳脱色工艺条件下，葵花籽蛋白的L*值为86.3、a*值为2.6、b*值为10.7，脱色葵花籽蛋白为浅白色，质地柔软细腻，脱色效果显著，其蛋白质含量和得率分别为97.50%和8.50%。 |
| 英文摘要: |
| The color of sunflower seed protein extracted by conventional method is dark gray, which seriously affects its application in food. Therefore, the bleaching process of sunflower seed protein was studied in order to prepare light color sunflower seed protein. Low temperature defatted sunflower seed meal was used as raw material, and the effect of limited enzymatic hydrolysis and macroporous resin on the bleaching of sunflower seed protein was investigated. Taking adsorption temperature, pH, adsorption time and resin amount as factors, and L* value of sunflower seed protein as evaluation index, orthogonal experiment was conducted. The results showed that the best bleaching effect of sunflower seed protein was obtained by limited enzymatic hydrolysis with Alcalase combined with adsorption by AB-8 macroporous resin. The optimal bleaching conditions of sunflower seed protein were pH 7.0, adsorption temperature 20 ℃, adsorption time 120 min and resin amount 12%. Under these conditions, the L* value, a* value and b* value of bleached sunflower seed protein were 86.3, 2.6 and 10.7, respectively. The bleached sunflower seed protein was light white, soft and delicate, and the bleaching effect was remarkable. The protein content and yield were 97.50% and 8.50%, respectively. |
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