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| 酶促酸解制备1-油酸-2-棕榈酸-3-亚油酸 甘油三酯 |
| Preparation of 1-oleoyl-2-palmitoyl-3-linoleoglglycerol by enzymatic acidolysis |
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| DOI： |
| 中文关键词: 1-油酸-2-棕榈酸-3-亚油酸甘油三酯 人乳脂 酸解反应 |
| 英文关键词:1-oleoyl-2-palmitoyl-3-linoleoglglycerol human milk fat acidolysis reaction |
| 基金项目:河南省重点研发与推广（科技攻关）专项（202102110288）；河南工业大学高层次人才科研启动基金项目（31401197） |
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| 中文摘要: |
| 以棕榈硬脂为原料富集高纯度三棕榈酸甘油三酯，以米糠油水解的游离脂肪酸为酰基供体，在脂肪酶的作用下采用一步酸解法制备1-油酸-2-棕榈酸-3-亚油酸甘油三酯（OPL），在单因素试验的基础上采用正交试验对OPL制备工艺条件进行优化。结果表明，OPL最佳制备工艺条件为：以Lipozyme RM IM为催化用酶，反应温度60 ℃，加酶量为底物总质量的10%，三棕榈酸甘油三酯与游离脂肪酸摩尔比1∶ 10，反应时间4 h。在最佳条件下，产物中棕榈酸分布在sn-2位的含量为52.02%，油酸和亚油酸分布在sn-1,3位的含量分别为33.21%和26.60%，推测产物中主要为OPL。 |
| 英文摘要: |
| Palm stearin was used as raw material to obtain the high purity of tripalmitin, and free fatty acid from rice bran oil was used as acyl donor, both of which were used as substrates for one-step acidolysis catalyzed by lipase to prepare 1-oleoyl-2-palmitoyl-3-linoleoglglycerol (OPL). On the basis of single factor experiment, orthogonal experiment was carried out to optimize the preparation of OPL. The optimal preparation conditions of OPL were obtained as follows: reaction temperature 60 ℃, with Lipozyme RM IM as catalyst, enzyme dosage 10% (based on the total mass of substrates), molar ratio of tripalmitin to free fatty acid 1∶ 10, reaction time 4 h. Under these conditions, palmitic acid located at sn-2 position accounted for 52.02%, and the contents of oleic acid and linoleic acid located at sn-1,3 positions were 33.21% and 26.60% respectively in the product. It could be inferred that OPL was the main product. |
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