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Preparation of 1-oleoyl-2-palmitoyl-3-linoleoglglycerol by enzymatic acidolysis
  
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KeyWord:1-oleoyl-2-palmitoyl-3-linoleoglglycerol  human milk fat  acidolysis reaction
FundProject:河南省重点研发与推广（科技攻关）专项（202102110288）；河南工业大学高层次人才科研启动基金项目（31401197）
Author NameAffiliation
SUN Cong College of Food Science and Engineering，Henan University of Technology，Zhengzhou 450001，China 
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Abstract:
      Palm stearin was used as raw material to obtain the high purity of tripalmitin, and free fatty acid from rice bran oil was used as acyl donor, both of which were used as substrates for one-step acidolysis catalyzed by lipase to prepare 1-oleoyl-2-palmitoyl-3-linoleoglglycerol (OPL). On the basis of single factor experiment, orthogonal experiment was carried out to optimize the preparation of OPL. The optimal preparation conditions of OPL were obtained as follows: reaction temperature 60 ℃, with Lipozyme RM IM as catalyst, enzyme dosage 10% (based on the total mass of substrates), molar ratio of tripalmitin to free fatty acid 1∶ 10, reaction time 4 h. Under these conditions, palmitic acid located at sn-2 position accounted for 52.02%, and the contents of oleic acid and linoleic acid located at sn-1,3 positions were 33.21% and 26.60% respectively in the product. It could be inferred that OPL was the main product.
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