In order to improve the purity and yield of the nervonic acid enriched from Acer truncatum Bunge. seed oil, the Acer truncatum Bunge. seed oil was saponified to prepare mixed fatty acids, and then the mixed fatty acids were dissolved in different solvents and crystallized at low temperature. The conditions of crystallization solvent type, ratio of mixed fatty acids to solvent and crystallization time were optimized. The results showed that when the mixed fatty acids were mixed with ethanol in a mass-volume ratio of 1∶ 3, and crystallized for 4 h at -40 ℃, the content of nervonic acid in the crystal was 13.11%, the yield was 65.48%, and the content of nervonic acid in the final product was more than twice as high as that in Acer truncatum Bunge. seed oil. |