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| 酶催化桑蚕蛹油酯交换制备中长链脂肪酸甘油酯 |
| Preparation of medium and long-chain fatty acid triglycerides from silkworm pupa oil by enzymatic interesterification reaction |
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| DOI： |
| 中文关键词: 中长链脂肪酸甘油三酯 桑蚕蛹油 脂肪酶 酯交换 |
| 英文关键词:medium and long-chain fatty acid triglycerides silkworm pupa oil lipase interesterification |
| 基金项目:广东省科技计划项目（2015B020234006，2017A070 702007）；广东省现代农业产业技术体系创新团队（2016LM 2151） |
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| 中文摘要: |
| 以桑蚕蛹油为底物，通过酶法催化其与三辛酸甘油酯进行酯交换反应，制备富含α-亚麻酸的中长链脂肪酸甘油三酯(MLCTs)。通过单因素实验考察了酶种类、底物质量比、反应温度、加酶量以及反应时间对酯交换反应的影响，对酯交换条件进行了优化。结果表明，最佳反应条件为：采用Lipozyme TL IM脂肪酶，三辛酸甘油三酯与桑蚕蛹油质量比1∶ 4，加酶量为底物质量的8%，反应温度45 ℃，反应时间10 h。在最佳反应条件下，酯交换反应的转化率为98.42%，酯交换产物中中长链脂肪酸甘油三酯含量为98.73%，辛酸含量为20.00%，α-亚麻酸含量为30.09%。 |
| 英文摘要: |
| Using silkworm pupa oil and caprylic triglyceride as substrates, medium and long-chain fatty acid triglycerides（MLCTs）rich in α-linolenic acid were prepared by enzymatic interesterification reaction. The effects of enzyme type, substrate mass ratio, reaction temperature, enzyme dosage and reaction time were studied by single factor experiment to optimize the reaction conditions. The results showed that the optimal reaction conditions were obtained as follows: using Lipozyme TL IM lipase, mass ratio of caprylic triglyceride to silkworm pupa oil 1∶ 4, enzyme dosage 8% of the substrate mass, reaction temperature 45 ℃ and reaction time 10 h. Under these conditions, the conversion rate of the interesterification reaction was 98.42%, and the contents of MLCTs, octanoic acid and α-linolenic acid in the interesterification products were 98.73%, 20.00% and 30.09%, respectively. |
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