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Preparation of medium and long-chain fatty acid triglycerides from silkworm pupa oil by enzymatic interesterification reaction
  
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KeyWord:medium and long-chain fatty acid triglycerides  silkworm pupa oil  lipase  interesterification
FundProject:广东省科技计划项目（2015B020234006，2017A070 702007）；广东省现代农业产业技术体系创新团队（2016LM 2151）
Author NameAffiliation
LIU Chen College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China； 
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Abstract:
      Using silkworm pupa oil and caprylic triglyceride as substrates, medium and long-chain fatty acid triglycerides（MLCTs）rich in α-linolenic acid were prepared by enzymatic interesterification reaction. The effects of enzyme type, substrate mass ratio, reaction temperature, enzyme dosage and reaction time were studied by single factor experiment to optimize the reaction conditions. The results showed that the optimal reaction conditions were obtained as follows: using Lipozyme TL IM lipase, mass ratio of caprylic triglyceride to silkworm pupa oil 1∶ 4, enzyme dosage 8% of the substrate mass, reaction temperature 45 ℃ and reaction time 10 h. Under these conditions, the conversion rate of the interesterification reaction was 98.42%, and the contents of MLCTs, octanoic acid and α-linolenic acid in the interesterification products were 98.73%, 20.00% and 30.09%, respectively.
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