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| Screening of sporulation medium for Mortierella alpina |
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| DOI： |
| KeyWord:：Mortierella alpina mycelium growth sporulation culture medium |
| FundProject:国家自然科学基金青年基金（31901659） |
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| Abstract: |
| In order to promote the sporulation of Moritierella alpina, the effects of eight mediums commonly used to promote the sporulation of filamentous fungi on Mortierella alpina mycelium growth and spore production were analyzed, and the sporulation medium was improved. The results showed that the average spore production in 40 g/L corn flour medium supplemented with 10 g/L glucose and inorganic salts (nitrate (2 g/L KNO3), phosphate (1 g/L NaH2PO4) and magnesium ion (0.3 g/L MgSO4·7H2O)) after incubation at 28 ℃ for 10 d and then at 4 ℃ for 14 d reached 8.57×106 spores/mL, which was 2.23 times as high as that in the glucose yeast medium (GY) (3.85×106 spores/mL) currently used for the preservation of Mortierella alpina strains. Therefore, 40 g/L of corn flour was the most suitable for the sporulation of Mortierella alpina in the presence of a small amount of glucose and inorganic salts to promote mycelium growth. |
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