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| Preparation of high purity DHA ethyl ester by molecular distillation and preparative liquid chromatography |
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| KeyWord:DHA-EE high purity molecular distillation preparative liquid chromatograph |
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| Abstract: |
| The separation and purification process of decosahexaenoic acid (DHA) in algae oil were discussed, and high purity DHA ethyl ester (DHA-EE) was obtained by improving the process conditions. Using ethylated algae oil as the raw material, high purity DHA-EE was prepared by molecular distillation and preparative liquid chromatography, and the process conditions were optimized by single factor experiment. The results showed that the optimal molecular distillation conditions were obtained as follows: evaporation temperature 105?℃, internal condensation temperature 55?℃, external condensation temperature 30?℃, material temperature 30?℃, rotation speed 160 r/min, vacuum degree 1 Pa, and feed rate 1 g/min. Under these conditions, the purity of DHA-EE was 72.1%, and the recovery rate was 716%. The optimal preparative liquid chromatography conditions were obtained as follows: using YMC-PACK ODS-A preparation column(5 μm,21.1 mm×250 mm), with methanol-water (volume ratio 95∶?5) as mobile phase, flow rate 20 mL/min, loading amount 0.2 g and detection wavelength 204 nm. Under these conditions, the DHA-EE purity reached more than 96%, and the average recovery rate was 79.66%. |
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