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| Process optimization for extraction and purification of filbert protein |
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| DOI： |
| KeyWord:Key words：filbert protein enzymolyze alcalase extraction purification |
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| Abstract: |
| Abstract：Filbert meal was used as raw materials to extract protein by alcalase assisted alkali solution and acid precipitation, then by adding alkali solution to reconstitute, alcalase to enzymolyze and acid to precipitate,filbert protein was purified.Based on the single factor experiment, the response surface methodology was used to optimize the enzymolysis process conditions in the extraction, and the orthogonal experiment was used to optimize the purification conditions of filbert protein.The results showed that the optimal enzymolysis conditions were obtained as follows: enzymolysis temperature 46 ℃, pH 8 and enzymolysis time 3.7 h, and the extraction rate of filbert protein reached 53.16% under these conditions. The optimal purification conditions were obtained as follows: reaction temperature 50 ℃, liquid to material ratio 5∶ 1, reconstitution pH 11, and the purity of filbert protein was 95.23% under the optimal conditions. |
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