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| Determination of benzo (a) pyrene in vegetable oil by high performance liquid chromatography with freeze-centrifugation purification |
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| KeyWord:Key words：benzo (a) pyrene high performance liquid chromatography vegetable oil freeze-centrifugation purification |
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| Abstract: |
| Abstract：A method was developed for the determination of benzo(a)pyrene in vegetable oil using freeze-centrifugation purification and high performance liquid chromatography. The sample was extracted with acetonitrile and acetone (volume ratio 4∶ 6) ,and then cleaned up by freeze-centrifugation. Benzo(a)pyrene was separated on a Kromasil C18 column,using a mobile phase consisting of acetonitrile and water (volume ratio 9∶ 1) at a flow rate of 1.0 mL/min. The excitation wavelength and emission wavelength were set at 365 nm and 406 nm respectively, and benzo (a) pyrene was detected with fluorescence detector. The results showed that the calibration curve showed good linearity in the range of 0.2-20 μg/L and the correlation coefficient was 0.999 7. The recoveries of benzo(a)pyrene in vegetable oil were in the range of 81.3%-90.8% at low, middle and high levels with RSD 1.35%-2.97%.The limit of detection(LOD) was 0.13 μg/kg and the limit of quantitation(LOQ) was 0.5 μg/kg. The established method was accurate, simple and suitable for the determination of benzo (a) pyrene in vegetable oil. |
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