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| 酸热预处理辅助水酶法提取牡丹籽油 |
| Acid-heat pretreatment assisted aqueous enzymatic extraction of peony seed oil |
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| DOI： |
| 中文关键词: 牡丹籽油 酸热预处理 水酶法 微观结构 |
| 英文关键词:peony seed oil acid-heat pretreatment aqueous enzymatic method microstructure |
| 基金项目:国家食品与工程一流学科建设项目(JUFSTR 20180202) |
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| 中文摘要: |
| 以脱壳牡丹籽为原料，研究牡丹籽经柠檬酸溶液浸泡、烘干及水酶法提取条件等对牡丹籽油提取率的影响。结果表明，牡丹籽油最佳提取条件为：牡丹籽经0.3 mol/L的柠檬酸溶液浸泡6 h，100 ℃烘干；在复合酶添加量0.5%、料液比1∶ 5、pH 4.5、50 ℃的条件下酶解1 h，再于pH 9、50 ℃下提取1 h，离心收集清油后，在pH 9、50 ℃条件下水相重复提取渣相1 h；选择木瓜蛋白酶破除乳状液。在上述条件下，牡丹籽清油提取率为55.31%，破乳率为96.31%，总油提取率为90.81%，所得牡丹籽油酸值、过氧化值等指标均达到牡丹籽油标准。通过激光共聚焦显微镜发现，牡丹籽经酸热预处理导致细胞壁变薄，蛋白质皱缩，油滴聚集。因此，酸热预处理辅助水酶法提取工艺在牡丹籽油的提取方面具有广阔的发展前景。 |
| 英文摘要: |
| With dehulled peony seed as raw material, the effects of soaking peony seeds in citric acid solution, drying, and aqueous enzymatic extraction conditions on the extraction rate of peony seed oil were studied. The results showed that the optimal extraction conditions were obtained as follows:soaking peony seeds in 0.3 mol/L citric acid solution for 6 h and drying at 100 ℃, and enzymolysis for 1 h at solid-liquid ratio 1∶ 5, 50 ℃ and pH 4.5 with 0.5% complex enzyme, then extraction for 1 h at pH 9 and 50 ℃, repeated extraction of slag phase in the same condition with water phase after collecting free oil by centrifugation, breaking emulsion with papain. Under these conditions, the demulsification rate was 96.31%, and the extraction rates of free oil and total oil were 55.31% and 90.81% respectively. The acid value and peroxide value of peony seed oil could meet the standard of peony seed oil. The laser confocal microscopy revealed that acid-heat pretreatment caused cell walls thinning, protein shrinkage, and oil droplet aggregation. So, acid-heat pretreatment assisted aqueous enzymatic extraction of peony seed oil had broad development prospects in the peony seed oil extraction. |
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