| The antimicrobial peptide was prepared from defatted Prinsepia utilis Royle meal by compound enzyme method. The best compound hydrolase types and ratio were screened. Based on single factor experiment, taking the inhibition rate of the hydrolysate of Prinsepia utilis Royle protein against Staphylococcus aureus as the response value, the enzymatic preparation process of hydrolysate of Prinsepia utilis Royle protein was optimized using response surface methodology. The results showed that the optimal process conditions were obtained as follows: compound protease and papain mixed in a ratio of 2∶ 1, enzyme addition amount 4.5%, enzymolysis temperature 60 ℃, material-liquid ratio 1∶ 35, pH 69, and enzymolysis time 4.7 h. Under these conditions, the hydrolysis degree of the Prinsepia utilis Royle protein was 23.92%, and the antibacterial rate of the hydrolysate of Prinsepia utilis Royle protein was 31.62%. The study provided a reference for the subsequent separation, identification and application of antimicrobial peptides from Prinsepia utilis Royle, and was of great significance to the comprehensive utilization of the by-products of Prinsepia utilis Royle. |
