| High-temperature sesame meal was used as the raw material, and the sesame protein was obtained by alkali-soluble and acid-precipitation, then low strength subcritical water was used to extract water-soluble protein and peptide. The effects of temperature, pH, reaction time and ratio of liquid to material on the yields of water-soluble protein and peptide were studied by single factor experiment, and the extraction process parameters of water-soluble protein and peptide were optimized by orthogonal experiment. The result showed that the yield of water-soluble protein and peptide could be significantly improved by low-strength subcritical water. The optimal process parameters for low strength subcritical water extraction of water-soluble protein were as follows: temperature 125 ℃, pH 1.5, reaction time 30 min,ratio of liquid to material 30∶ 1. Under the optimal conditions,the yield of water-soluble proteins was up to 85.015%. The optimal process parameters for subcritical water extraction of peptide were as follows: hydrolysis temperature 125 ℃, pH 1.5, reaction time 60 min, ratio of liquid to material 50∶ 1. Under the optimal conditions, the yield of peptide was up to 40537%. The extraction rate of subcritical water was high, the operation was simple, and it provided a reference for the extraction of water-soluble sesame protein and peptide. |
