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| 棉籽中黄酮的提取、纯化及主要成分分析 |
| Extraction，purification and main components analysis of cottonseed flavonoids |
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| DOI： |
| 中文关键词: 棉籽 黄酮类化合物 大孔吸附树脂 UPLC-Q-TOF-MS |
| 英文关键词:cottonseed flavonoids macroporous adsorption resin UPLC-Q-TOF-MS |
| 基金项目:内蒙古农业大学学校引进项目（DC1900007017） |
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| 中文摘要: |
| 以脱脂棉籽粕为原料，采用溶剂法提取其中的黄酮，并用大孔吸附树脂进行纯化。以超高效液相色谱-串联四级杆-飞行时间质谱（UPLC-Q-TOF-MS）联用技术对棉籽黄酮的化学成分进行分析与鉴定。结果表明：棉籽黄酮的最佳提取条件为70%乙醇作为提取溶剂，料液比1∶ 8，提取温度25 ℃，提取时间5 h；大孔吸附树脂纯化的最优条件为选用D101大孔吸附树脂，样品溶液pH 4，解吸剂为70%乙醇，解吸液流速为2 BV/h。在最佳提取条件下，所得粗棉籽黄酮的纯度为23.4%。在最佳纯化条件下，棉籽黄酮的纯度提高到57.9%。棉籽黄酮中共鉴定出黄酮类化合物25种，以槲皮素和山奈酚黄酮骨架为主。 |
| 英文摘要: |
| Defatted cottonseed meal was used as the material, and the flavonoids were extracted with solvent and purified with macroporous adsorption resin. The chemical constituents of cottonseed flavonoids were analysed and identified by ultra performance liquid chromatography-tandem quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS). The results showed that the optimal conditions for extracting flavonoids from deffatted cottonseed meal with solvent method were obtained as follows: solvent (ethanol) volume fraction 70%, material-liquid ratio 1∶ 8,extraction temperature 25 ℃, extraction time 5 h. Under the optimal conditions,the purity of the crude cottonseed flavonoids was 23.4%.The optimal purification conditions with macroporous adsorption resin were obtained as follows: D101 resin, pH of the sample solution 4, 70% ethanol used as the desorption solution, desorption solution flow rate 2 BV/h. Under these conditions, the purity of the purified cottonseed flavonoids was 57.9%. A total of 25 flavonoids were identified, dominated by quercetin and kaempferol flavonoids. |
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