|
| Extraction，purification and main components analysis of cottonseed flavonoids |
| |
| DOI： |
| KeyWord:cottonseed flavonoids macroporous adsorption resin UPLC-Q-TOF-MS |
| FundProject:内蒙古农业大学学校引进项目（DC1900007017） |
|
| Hits: 978 |
| Download times: 523 |
| Abstract: |
| Defatted cottonseed meal was used as the material, and the flavonoids were extracted with solvent and purified with macroporous adsorption resin. The chemical constituents of cottonseed flavonoids were analysed and identified by ultra performance liquid chromatography-tandem quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS). The results showed that the optimal conditions for extracting flavonoids from deffatted cottonseed meal with solvent method were obtained as follows: solvent (ethanol) volume fraction 70%, material-liquid ratio 1∶ 8,extraction temperature 25 ℃, extraction time 5 h. Under the optimal conditions,the purity of the crude cottonseed flavonoids was 23.4%.The optimal purification conditions with macroporous adsorption resin were obtained as follows: D101 resin, pH of the sample solution 4, 70% ethanol used as the desorption solution, desorption solution flow rate 2 BV/h. Under these conditions, the purity of the purified cottonseed flavonoids was 57.9%. A total of 25 flavonoids were identified, dominated by quercetin and kaempferol flavonoids. |
| View full text View/Add Comment Download reader |
| Close |
|
|
|
