| Soybean dregs are one of the main by-products of soybean processing, and are rich in protein and dietary fiber. The protein peptides and soluble dietary fiber (SDF) were separately extracted from soybean dregs by combined enzymatic method. First, the protein in the soybean dregs was hydrolyzed with alcalase, and the yield of protein peptides was used as an indicator,the conditions for extracting protein peptides from soybean dregs were optimized through single factor experiment, and then the deproteinized soybean dregs were hydrolyzed with cellulase to prepare SDF, and the yield of SDF was used as an indicator, the process conditions for extracting SDF were optimized through single factor experiment. The results showed that the optimal conditions for extracting protein peptides from soybean dregs were obtained as follows: material-liquid ratio 1∶ 35, enzyme-substrate ratio (E/S)2%, enzymolysis temperature 50 ℃, pH 9.5, and enzymolysis time 5 h. Under the optimal conditions,the yield of protein peptides was 66.81%.The optimal conditions for extracting SDF from deproteinized soybean dregs were obtained as follows: material-liquid ratio 1∶ 30, E/S 3%, enzymolysis temperature 50 ℃, enzymolysis time 2 h, and pH 40. Under the optimal conditions,the yield of SDF was 1554%. After enzymolysis with alcalase and cellulase sequentially, the total utilization rate of soybean dregs reached 89.81%, which provided a new way for the comprehensive development and utilization of soybean dregs. |
