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| Comparison of three chromatographic columns separating various fatty acid methyl esters |
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| DOI： |
| KeyWord:fatty acid fatty acid methyl ester chromatographic column separation effect |
| FundProject:深圳市卫生计生系统科研项目（SZFZ2017027, 201606043）；深圳市科创委基础研究项目（JCYJ2017 0307144652484）；国家卫生健康委员会食物成分监测项目；深圳市医疗卫生三名工程项目（SZSM201611017） |
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| Abstract: |
| The results of three chromatographic columns separating various fatty acid methyl esters (FAME) standards were compared for the reference of detecting fatty acids quickly and accurately. The methods of separating 37 FAME standards with HP-88, SP-2560 and DB-23 were established and used to analyze 13 trans FAME standards, 4 isomers of linoleic acid methyl ester and 8 isomers of linolenic acid methyl ester. The results showed that the analysis time of three columns separating 37 FAME was 47, 35 min and 25 min respectively, and all of the resolutions were greater than 1.0. The cis-trans monounsaturated fatty acid methyl ester and linoleic acid methyl ester isomer could be separated by the three columns. HP-88 was better than SP-2560 and DB-23 in the separation of linolenic acid methyl ester and isomers of fatty acid with double bonds at different positions, but neither of them met the requirements of quantitative analysis. Suitable chromatographic columns could be selected according to the types of fatty acids in the samples to avoid the overlapping of chromatographic peaks in actual sample analysis. |
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