Optimization of extraction process of Moringa oleifera seed protein by reverse micelle method
  
DOI:
KeyWord:Moringa oleifera seed  Moringa oleifera seed protein  reverse micelle  pre-extraction  post-extraction
FundProject:国家自然科学基金地区项目(31960462);云南省基础研究计划面上项目(2019FB052);云南省农业联合专项(2018FG001-040)
Author NameAffiliation
HE Li College of Food Science and Technology Yunnan Agricultural University Kunming 650201 China 
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Abstract:
      Using defatted Moringa oleifera seed powder as raw material, the reverse micelle method was used to extract Moringa oleifera seed protein and the process was optimized. Through single factor experiment and orthogonal experiment, the effects of the reverse micelle (AOT)mass concentration, extraction temperature, pH, ratio of material to liquid, and water activity on the reverse micelle pre-extraction process for extracting Moringa oleifera seed protein were investigated. The single factor experiment and the second general rotating experiment were used to investigate the ultrasound-assisted reverse micelle post-extraction process of extracting Moringa oleifera seed protein with four factors of extraction temperature, pH, KCl concentration, and extraction time. The results showed that the optimal process conditions for the reverse micelle pre-extraction were obtained as follows: pH 9, ratio of material to liquid 1∶ 50, AOT mass concentration 0.08 g/mL, extraction temperature 45 ℃, water activity 25. Under these conditions, the extraction rate of pre-extracted Moringa oleifera seed protein was 67.2%. The optimal process conditions for ultrasound-assisted reverse micelle post-extraction were obtained as follows: extraction time 45 min, extraction temperature 45 ℃, pH 6.5, and KCl concentration 1.25 mol/L. Under these conditions, the extraction rate of post-extracted Moringa oleifera seed protein was 585%.
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