| Using defatted Moringa oleifera seed powder as raw material, the reverse micelle method was used to extract Moringa oleifera seed protein and the process was optimized. Through single factor experiment and orthogonal experiment, the effects of the reverse micelle (AOT)mass concentration, extraction temperature, pH, ratio of material to liquid, and water activity on the reverse micelle pre-extraction process for extracting Moringa oleifera seed protein were investigated. The single factor experiment and the second general rotating experiment were used to investigate the ultrasound-assisted reverse micelle post-extraction process of extracting Moringa oleifera seed protein with four factors of extraction temperature, pH, KCl concentration, and extraction time. The results showed that the optimal process conditions for the reverse micelle pre-extraction were obtained as follows: pH 9, ratio of material to liquid 1∶ 50, AOT mass concentration 0.08 g/mL, extraction temperature 45 ℃, water activity 25. Under these conditions, the extraction rate of pre-extracted Moringa oleifera seed protein was 67.2%. The optimal process conditions for ultrasound-assisted reverse micelle post-extraction were obtained as follows: extraction time 45 min, extraction temperature 45 ℃, pH 6.5, and KCl concentration 1.25 mol/L. Under these conditions, the extraction rate of post-extracted Moringa oleifera seed protein was 585%. |
