| In order to establish a pretreatment method for the determination of benzo(a) pyrene in vegetable oil with high accuracy and low cost, based on GB 5009.7-2016, a self-made aluminum oxide column was used as a SPE column,five factors affecting the elution of benzo (a) pyrene in oil were studied by single factor experiment, including sample amount, elution solvent dosage, elution flow rate, adsorbent storage time and adsorbent dosage, to optimize the pretreatment conditions. In addition, the detection method was evaluated. The results showed that the optimal pretreatment conditions were obtained as follows: sample amount 0.100 0 g, elution solvent dosage 120 mL, adsorbent(aluminum oxide) dosage 22 g, elution flow rate 1 drop /2 s, and adsorbent storage time not exceeding 12 weeks. The detection limit of the method was 0.2 μg/L, the recovery rate of spiked standard was 94.23%-100.00%, and the RSD was 1.20%-7.36%. The results of the same sample were close to those of SGS with a relative mean deviation of 2.36%-3.50%. The above results indicated that the method was accurate and could be applied to the determination of trace benzo (a) pyrene in vegetable oil in oil enterprises. |
