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| Determination of contents of ALA, EPA and DHA in the yolk of ω-3 nutrient-fortified egg by gas chromatography |
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| DOI： |
| KeyWord:ω-3 polyunsaturated fatty acid ALA EPA DHA egg yolk gas chromatography internal standard method |
| FundProject:国家重点研发计划（2018YFC1602300） |
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| Abstract: |
| A rapid and accurate method for determination of ALA, EPA and DHA in ω-3 nutrient-fortified egg yolk by gas chromatography was developed. The samples were firstly methylated with acetyl chloride-methanol, then determined by gas chromatography. The fatty acid methyl esters were separated by Agilent DB-FastFAME column and quantified with triundecanoin as internal standard. The results showed that internal standard substance, ALA, EPA and DHA were well separated from each other. The ALA, EPA and DHA had a good linear relationship between their mass concentrations and chromatographic peak areas (R2≥0.99). The recoveries rate were 87.2%-101.1% with RSD 1.1%-2.6%. This method was simple, fast and accurate, and suitable for batch determination of ω-3 PUFA in the yolk of ω-3 nutrient-fortified eggs. |
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