|
| Preparation of triglyceride fish oil catalyzed by immobilized lipase |
| |
| DOI： |
| KeyWord:lipase fish oil glycerides transesterification |
| FundProject:浙江省重点研发计划（2019C01082） |
|
| Hits: 1209 |
| Download times: 548 |
| Abstract: |
| With immobilized lipase as catalyst, the triglyceride fish oil was prepared from fish oil ethyl esters and glycerol by transesterification. The effects of enzyme species, vacuum degree, reaction temperature, enzyme dosage on transesterification were investigated by single factor experiment, and the transesterification conditions were optimized by orthogonal experiment. The results showed that the optimal conditions of preparing triglyceride fish oil catalyzed by immobilized lipase were obtained as follows:Novozym 435 lipase as catalyst, vacuum degree 500 Pa, reaction temperature 50 ℃, enzyme dosage 2%, reaction time 15 h. Under these conditions, the triglyceride content reached 78%, and it could reach 82.08% after molecular distillation. The triglyceride content could reach 74% when the immobilized lipase was used for fifteen times. |
| View full text View/Add Comment Download reader |
| Close |
|
|
|
