| Lacquer seed oil was extracted from kernel of lacquer seeds from Qinba Mountain by solvent(petroleum ether) extraction.On the basis of single factor experiment, response surface methodology was used to optimize the extraction process of lacquer seed oil. The physicochemical indexes of lacquer seed oil were analyzed,and the fatty acid composition of lacquer seed oil was analyzed by GC-MS, its antioxidant activity was also evaluated. The results showed that the optimal extraction process of lacquer seed oil were obtained as follows: extraction temperature 70 ℃, extraction time 60 min, liquid-material ratio 10∶ 1, extraction times twice.Under the optimal conditions,the extraction rate of lacquer seed oil was 96.14%.The relative density, refractive index (20 ℃), iodine value,acid value, peroxide value and saponification value of lacquer seed oil were 0.925 8, 1.467 2, 146.83 gI/100 g, 2.58 mgKOH/g, 3.62 mmol /kg and 185.36 mgKOH/g, respectively. Lacquer seed oil mainly contained six kinds of fatty acids including oleic acid, linoleic acid, palmitic acid, stearic acid, linolenic acid and palmitoleic acid, and the content of unsaturated fatty acids reached up to 86.10%, in which the content of oleic acid and linoleic acid reached 82.17%. The IC50 of lacquer seed oil for DPPH, ABTS and hydroxyl radicals were 35.38, 41.29 mg/L and 38.52 mg/L, showing equivalent antioxidant activity with vitamin C. |
