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| 南极磷虾油磷脂富集工艺的优化 |
| Optimization of enrichment process of phospholipids in Antarctic krill oil |
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| DOI： |
| 中文关键词: 南极磷虾油 磷脂富集 甘油三酯 |
| 英文关键词:Antarctic krill oil phospholipids enrichment triglyceride |
| 基金项目:国家重点研发计划（2018YFC1406806） |
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| 中文摘要: |
| 借鉴水化脱胶原理，对南极磷虾油磷脂富集工艺进行优化。通过单因素实验考察了反应溶液类型、质量分数、加入量，反应温度，反应时间及搅拌速度对南极磷虾油磷脂富集效果及富集后各功能成分的影响，得到南极磷虾油磷脂富集的最佳工艺条件为南极磷虾油中加入4倍南极磷虾油磷脂量的2%柠檬酸溶液、反应温度60 ℃、搅拌速度60 r/min、反应时间30 min。在最佳条件下磷脂富集效果明显，分离得到的磷脂溶液经冻干后磷脂含量可达70.78%，EPA和DHA含量分别为151.93 mg/g和113.70 mg/g；而分离得到的甘油三酯中虾青素含量最高可达780.49 mg/kg。 |
| 英文摘要: |
| Based on the principle of hydration degumming, the enrichment process of phospholipids in Antarctic krill oil was optimized. The effects of types, mass fraction and amount of reaction solution, reaction temperature, reaction time and stirring speed on the enrichment effect of phospholipid in krill oil and the functional components after enrichment were investigated by single factor experiment. The optimal process conditions for the enrichment of phospholipids in krill oil were obtained as follows: adding 2% citric acid solution with four times amount of phospholipids in the krill oil, reaction temperature 60 ℃, stirring speed 60 r/min, and reaction time 30 min. Under these conditions, the enrichment effect of phospholipids was obvious, and the lyophilized phospholipid solution contained 70.78% phospholipids, 151.93 mg/g EPA and 113.70 mg/g DHA respectively, and the highest content of astaxanthin in the triglyceride was 780.49 mg/kg. |
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