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| 超声辅助酶解法提取羊油工艺优化 |
| Ultrasound-assisted enzymatic hydrolysis for extraction of sheep oil |
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| DOI： |
| 中文关键词: 羊油 超声辅助酶解法 Plackett-Burman试验 Box-Behnken试验 理化指标 |
| 英文关键词:sheep oil ultrasound-assisted enzymatic hydrolysis Plackett-Burman design Box-Behnken design physicochemical index |
| 基金项目:新疆维吾尔自治区自然科学基金项目 (2019D01A38) |
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| 中文摘要: |
| 以阿勒泰羊尾脂为试验材料，采用超声辅助中性蛋白酶酶解法提取羊油。在单因素试验的基础上，通过Plackett-Burman试验筛选出对羊油得率具有显著影响的因素，并在此基础上采用Box-Behnken设计响应面试验对羊油提取工艺进行优化。结果表明，超声辅助酶解法提取羊油最佳工艺条件为料液比1∶ 2、中性蛋白酶添加量1.0%、超声酶解温度46 ℃、酶解时间2 h、超声时间34 min、超声功率200 W，在此条件下羊油得率为79.98%，显著高于干法熬制（74.35%）和酶解法（72.16%）（P<0.05）。3种方法所得羊油熔点、皂化值和碘值差异不显著（P＞0.05）；超声辅助酶解法所得羊油过氧化值（0.38 mmol/kg）和酸值（KOH）（0.86 mg/g）显著低于干法熬制所得羊油，酸值（KOH）显著高于酶解法所得羊油（P＜0.05）。采用超声辅助酶解法提取羊油得率高，产品品质较好，为羊尾脂的加工利用提供新思路和一定的理论依据。 |
| 英文摘要: |
| The fat in Altay sheep’s tails was taken as test material, and sheep oil was extracted with ultrasound-assisted neutral protease enzymatic hydrolysis. On the basis of single factor experiment, the factors that had significant influence on the yield of sheep oil was selected through Plackett-Burman design, and on this basis, Box-Behnken design response surface methodology was used to optimize the extraction process of sheep oil. The results showed that the optimal process conditions were obtained as follows: ratio of material to liquid 1∶ 2, dosage of neutral protease 1.0%, ultrasonic enzymatic hydrolysis temperature 46 ℃, enzymatic hydrolysis time 2 h, ultrasonic time 34 min, and ultrasonic power 200 W. Under these conditions, the yield of sheep oil was 79.98%, which was significantly higher than dry decocting (74.35%) and enzymatic hydrolysis method (72.16%) (P<0.05). There was no significant difference in the melting point, saponification value and iodine value of the three methods (P>0.05). The peroxide value (0.38 mmol/kg) and acid value (0.86 mgKOH/g) of the sheep oil obtained by ultrasound-assisted enzymatic hydrolysis method were significantly lower than that of dry decocting, and the acid value was significantly higher than that of enzymatic hydrolysis (P<0.05). These results indicated that ultrasound-assisted enzymatic extraction process could increase the yield and improve the quality of sheep oil. This process could provide new ideas and theoretical basis for the processing and utilization of the fat in sheep’s tails. |
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