| Vicia faba protein was prepared from Vicia faba by alkaline solution and acid precipitation method, then enzymolyzed to prepare Vicia faba proteolysate with α-glucosidase inhibition.The effects of protease type, enzymolysis temperature, enzymolysis pH, solid-liquid ratio, enzyme dosage and enzymolysis time on the enzymatic hydrolysis of Vicia faba protein were investigated using α-glucosidase inhibition rate and hydrolysis degree as indicators. Then the process conditions of preparing proteolysate with α-glucosidase inhibition from Vicia faba protein were optimized by response surface methodology. The results showed that the optimal conditions of preparing Vicia faba proteolysate with α-glucosidase inhibition from Vicia faba protein were obtained as follows: with alkaline protease as enzyme, enzymolysis temperature 50 ℃, enzymolysis pH 8.5, enzymolysis time 4.3 h, solid-liquid ratio 1∶ 10, enzyme dosage 14 000 U/g.Under the optimal conditions, the α-glucosidase inhibition rate of the proteolysate was (38.58±0.87)%, and the hydrolysis degree of Vicia faba protein was 22.87%. |
