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| Preparation of hemp peptide-calcium chelate and its structural characterization and stability properties |
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| DOI： |
| KeyWord:hemp peptide peptide calcium chelate structure characterization stability |
| FundProject:湖南省教育厅科学研究项目（18C1827） |
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| Abstract: |
| Hemp peptide(HPIH) was prepared by defatting, protein extraction and enzymatic hydrolysis of hemp seed kernel, then chelated with calcium to prepare hemp peptide-calcium chelate(HPIH-Ca), and the calcium chelation rate was used as the index to optimize preparation parameters by single factor experiment and orthogonal experiment. The structure of the chelate was analyzed by infrared spectroscopy and circular dichroism, and the microstructure of the chelate was characterized by scanning electron microscopy. The stability of the chelate was evaluated by heat treatment, pH and simulated digestion in vitro. The results showed that trypsin was the best hydrolase, and the optimal process parameters were obtained as follows: ratio of peptide to calcium 2∶ 1 (volume ratio of 10 mg/mL HPIH solution to 0.05 mol/L anhydrous calcium chloride), pH 8.0, reaction temperature 60 ℃ and reaction time 45 min. Under these conditions, calcium chelation rate was 47.61 mg/g. The interaction between Ca2+ and hemp peptide was mainly through carboxylic oxygen and amino nitrogen atoms. The Ca2+ combined with HPIH to form a more ordered and compact secondary structure. HPIH was cross-linked with Ca2+ to form a large granular and lamellar complex with orderly and tight structure. HPIH-Ca had certain heat resistance but was unstable under acidic conditions. Simulated digestion in vitro results showed that HPIH-Ca chelate could maintain calcium retention rate of more than 70% and had high bioavailability. |
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