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| GPC-UPLC-MS/MS测定食用油脂中 5种辣椒素类物质 |
| Determination of five kinds of capsaicins in edible oil by GPC-UPLC-MS/MS |
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| DOI： |
| 中文关键词: 凝胶渗透色谱（GPC） 超高效液相色谱-串联质谱（UPLC-MS/MS） 辣椒素 食用油脂 |
| 英文关键词:gel permeation chromatography (GPC) ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) capsaicin edible oil |
| 基金项目:“十三五”国家重点研发计划项目(2017YFC1 601705)；安徽省重点研究与开发计划项目(202004a07020024) |
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| 中文摘要: |
| 建立了凝胶渗透色谱-超高效液相色谱-串联质谱法(GPC-UPLC-MS/MS)测定食用油脂中天然辣椒素、二氢辣椒素、合成辣椒素、降二氢辣椒素、高二氢辣椒素含量的方法。样品经凝胶渗透色谱在线净化后，采用超高效液相色谱-串联质谱法分析，动态多反应监测模式（dMRM），同位素内标法定量。结果表明：方法在0.05～10 μg/L范围内线性良好，线性相关系数均大于等于0.998；检出限为0.02~0.05 μg/kg，定量限为0.10 μg/kg；在0.20、1.00、5.00 μg/kg添加水平下，平均加标回收率为90.6％～101.5％，相对标准偏差（RSD）为2.5％～9.7％。该方法前处理过程简便快捷，定量准确，可满足实验室准确测定食用油脂中5种辣椒素类物质的需求。 |
| 英文摘要: |
| A new analytical method was developed for the simultaneous determination of capsaicin, dihydrocapsaicin, nonivamide, nordihydrocapsaicin and homodihydrocapsaicin in edible oil by gel permeation chromatography (GPC) coupled with ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The sample was cleaned by GPC online, then analyzed by UPLC-MS/MS based on dynamic multiple reaction monitoring (dMRM) mode, and quantified using isotope internal standard technique. The results showed that the method was linear in the range of 0.05-10 μg/L with linear correlation coefficients not less than 0.998, and the limits of detection and quantitation were 0.02-0.05 μg/kg and 0.10 μg/kg respectively. The average recoveries were in the range of 90.6%-101.5% at 0.20,1.00,5.00 μg/kg spiked levels with relative standards deviation (RSD) between 2.5% and 9.7%. The method had the items of simple, rapidness, and accuracy, and could be used to accurately quantify the five kinds of capsaicins in edible oil. |
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