|
| Detection of inositol during the preparation of inositol fatty acid esters |
| |
| DOI： |
| KeyWord:inositol inositol fatty acid esters high performance liquid chromatography with evaporative light scattering detector |
| FundProject:国家“十三五”重点研发项目“特色油料适度加工与综合利用技术及智能装备研发与示范”（ 2018YFD0401100） |
|
| Hits: 1017 |
| Download times: 638 |
| Abstract: |
| A reverse-phase high performance liquid chromatography with evaporative light scattering detector (HPLC-ELSD) method was developed for the analysis of inositol during the preparation of inositol fatty acid esters. The sample was extracted and diluted with water for subsequent analysis. The Agilent ZORBAX 300SB-C18 (4.6 mm×250 mm,5 μm) was utilized. The mobile phase was methanol solution containing 0.1% acetic acid and the flow rate was 0.8 mL/min. Evaporator temperature was 55 ℃ and N2 pressure was 206.84 kPa with the gain 10. Inositol was quantified by external standard method. The results showed that the method had good linearity for inositol in the range of 0.01-1.00 mg/mL and R2 was 0.993 4. The recoveries of inositol were in the range of 80%-108% with the relative standard deviation less than 6%. This method was simple, stable, sensitive and reliable. It could be applied to the determination of inositol during the preparation of inositol fatty acid esters. |
| View full text View/Add Comment Download reader |
| Close |
