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| 液液萃取-UPLC快速测定初榨橄榄油中叶绿素铜钠的含量 |
| Rapid determination of sodium copper chlorophyllin in virgin olive oil by liquid-liquid extraction and UPLC |
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| DOI： |
| 中文关键词: 叶绿素铜钠 液液萃取 超高效液相色谱法 初榨橄榄油 |
| 英文关键词:sodium copper chlorophyllin liquid-liquid extraction ultra-high performance liquid chromatography virgin olive oil |
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| 中文摘要: |
| 建立了液液萃取-超高效液相色谱（UPLC）快速测定初榨橄榄油中叶绿素铜钠含量的方法。初榨橄榄油样品经80%的乙醇溶液提取后，在UPLC条件为采用SVEA C18色谱柱（50 mm×1.0 mm, 1.7 μm），柱温30 ℃，乙腈-水（体积比95∶ 5）为流动相，等度洗脱，流速0.3 mL/min，进样量2.0 μL，检测波长405 nm下，可实现初榨橄榄油中叶绿素铜钠的有效分离，整个分析过程仅需6 min。通过方法学考察，该方法的检出限及定量限分别为0.20 mg/L和0.50 mg/L，叶绿素铜钠加标回收率均在84.00%~97.90%之间，方法的重复性、精密度和稳定性较好。所建立的方法可用于初榨橄榄油中叶绿素铜钠含量的快速测定。 |
| 英文摘要: |
| A method for rapid determination of sodium copper chlorophyllin in virgin olive oil by liquid-liquid extraction and ultra-high performance liquid chromatography (UPLC)was established. Virgin olive oil was extracted by 80% ethanol, then injected to ultra-high performance liquid chromatography, and determined under the conditions of SVEA C18 column (50 mm×1.0 mm, 1.7 μm) with isocratic elution at 30 ℃, acetonitrile-water (volume ratio 95∶ 5) as mobile phase, flow rate 0.3 mL/min, injection volume 2.0 μL, and detection wavelength 405 nm,the sodium copper chlorophyllin could be effectively separated from virgin olive oil,and the entire analysis process took only 6 min. The results of the methodological examination showed that the detection limit and quantitative limit of the method were 0.20 mg/L and 0.5 mg/L respectively, the recovery rate of sodium copper chlorophyllin was between 84.00% and 97.90%, and the method had better reproducibility, precision and stability,and could be used for the determination of sodium copper chlorophyllin in virgin olive oil. |
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