| The influencing factors of PLA1 enzymatic degumming were studied, and the optimal laboratory parameters of PLA1 enzymatic degumming were determined. Three degumming methods of PLA1, PLC and PLC combined with PLA1 double enzymes were tested on different batches of crude soybean oil. The results showed that the optimal conditions for the PLA1 enzymatic degumming were obtained as follows: enzyme dosage 20 mg/kg, water dosage 3%, stirring speed 500 r/min, reaction temperature 55 ℃ and reaction time 2 h. Under these conditions, the phosphorus content of soybean oil decreased to within 10 mg/kg. The oil yield increased respectively 0.74-0.91 percentage points by PLA1 enzymatic degumming, 0.70-0.84 percentage points by PLC enzymatic degumming, 1.34-1.89 percentage points by PLC combined with PLA1 enzymatic degumming. The pilot production showed that the oil yield of PLA1 and PLC single enzymes degumming increased by 063 percentage points and 0.91 percentage points, respectively compared with water deyumming. |
