| With fresh Larimichthys crocea fish as raw material, the Larimichthys crocea fish oil was extracted by petroleum ether (boiling range 30-60 ℃). The acid value, peroxide value, anisidine value, conjugated diene value, fatty acid composition, content of tocopherols, cholesterol, squalene, volatile components and phospholipid composition of Larimichthys crocea fish oil were determined. The results showed that the yield of Larimichthys crocea fish oil was (31.25±3.66)%, and the acid value, peroxide value, anisidine value and conjugated diene value were (0.59±005)mgKOH/g, (550±0.76) mmol/kg, 030±006 and 2232±1.09, respectively. A total of 17 fatty acids were detected in Larimichthys crocea fish oil, and the content of unsaturated fatty acids was 67.27%, in which the oleic acid, linoleic acid, DHA and EPA accounted for (31.03±010)%,(1221±0.07)%,(7.21±0.12)% and (4.48±003)%,respectively. There were four tocopherols in Larimichthys crocea fish oil,α-tocopherol content was 4.18 mg/100 g, and the contents of β-tocopherol, γ-tocopherol and δ-tocopherol were all below 0.12 mg/100 g. The contents of squalene and cholesterol in Larimichthys crocea fish oil were 40 mg/100 g and 380 mg/100 g, respectively. A total of 31 volatile components were identified in Larimichthys crocea fish oil, mainly including aldehydes, ketones, alcohols and alkanes. The high contents of phospholipids in Larimichthys crocea fish oil were PG (30∶ 0/16∶ 0) (19.61 mg/g) and PG (28∶ 0/16∶ 1) (10.87 mg/g). |
