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| 芥子酸对油酸诱导HepG2细胞脂毒性模型 差异表达基因的影响 |
| Effects of sinapic acid on differentially expressed genes in oleic acid-induced HepG2 cytotoxicity model |
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| DOI： |
| 中文关键词: 芥子酸 HepG2细胞 差异表达基因 菜籽油 |
| 英文关键词:sinapic acid HepG2 cell differentially expressed gene rapeseed oil |
| 基金项目:：国家重点研发计划（2017YFD0400200）；江苏特聘教授项目和“太湖人才计划”资助 |
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| 中文摘要: |
| 菜籽油富含酚酸，其中芥子酸具有较强的抗氧化能力，能够清除氧自由基，提高细胞氧化应激反应能力。以油酸诱导HepG2细胞构建脂毒性模型，通过RNA-seq分析芥子酸干预对HepG2细胞差异表达基因的影响，并对HepG2细胞中差异表达基因进行了GO分类和KEGG富集分析。结果表明：对照组、模型组、实验组、模型实验组间均存在差异表达基因，与对照组相比，模型组、模型实验组和实验组中下调基因多于上调基因。GO功能分类分析发现油酸和芥子酸主要从细胞过程、刺激反应、催化活性方面影响细胞物质代谢和酶的催化基因表达。在KEGG通路富集中，油酸和芥子酸干预改变了HepG2细胞相关有丝分裂原活化蛋白激酶信号通路、松弛素信号通路和白细胞介素17信号通路等多个通路，说明芥子酸对细胞有机系统有一定的影响，并且可以通过调节PPAR信号通路来刺激PPARγ的活性从而影响脂肪的代谢与沉积。 |
| 英文摘要: |
| Rapeseed oil is rich in phenolic acids, among which sinapic acid has a strong antioxidant ability. Sinapic acid can scavenge oxygen free radicals and improve the ability of oxidative stress response of cells. The RNA-seq technology was used to analyze the effect of sinapic acid on the differentially expressed genes in HepG2 cells based on the lipotoxicity model established by oleic acid. The classification of Gene Ontology(GO) and KEGG enrichment of differentially expressed genes in HepG2 cells were analyzed. The results showed that there were differentially expressed genes among control group, model group, experimental group and experimental group based on the model. Compared with the control group, the expression level of down-regulated genes was more than that of up-regulated genes in model group, experimental group based on the model and experimental group. The GO analysis showed that oleic acid and sinapic acid affected the cellular metabolism and the catalytic gene expression of enzymes mainly from the aspects of cellular process, response to stimulus and catalytic activity. In the analysis of the KEGG pathway enrichment, the intervention of oleic acid and sinapic acid changed the mitogen-activated protein kinase (MAPK) signaling pathway, relaxin signaling pathway and interleukin 17 signaling pathway in HepG2 cell, etc. These results indicated that sinapic acid had a certain effect on the cellular organic system, and could stimulate the activity of PPARγ by regulating the PPAR signaling pathway, thereby affecting the metabolism and deposition of fat. |
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