| Rapeseed oil is rich in phenolic acids, among which sinapic acid has a strong antioxidant ability. Sinapic acid can scavenge oxygen free radicals and improve the ability of oxidative stress response of cells. The RNA-seq technology was used to analyze the effect of sinapic acid on the differentially expressed genes in HepG2 cells based on the lipotoxicity model established by oleic acid. The classification of Gene Ontology(GO) and KEGG enrichment of differentially expressed genes in HepG2 cells were analyzed. The results showed that there were differentially expressed genes among control group, model group, experimental group and experimental group based on the model. Compared with the control group, the expression level of down-regulated genes was more than that of up-regulated genes in model group, experimental group based on the model and experimental group. The GO analysis showed that oleic acid and sinapic acid affected the cellular metabolism and the catalytic gene expression of enzymes mainly from the aspects of cellular process, response to stimulus and catalytic activity. In the analysis of the KEGG pathway enrichment, the intervention of oleic acid and sinapic acid changed the mitogen-activated protein kinase (MAPK) signaling pathway, relaxin signaling pathway and interleukin 17 signaling pathway in HepG2 cell, etc. These results indicated that sinapic acid had a certain effect on the cellular organic system, and could stimulate the activity of PPARγ by regulating the PPAR signaling pathway, thereby affecting the metabolism and deposition of fat. |
