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| Effect of palmitic acid on lipid metabolism of human normal liver cells based on HPLC-MS lipidomics |
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| DOI： |
| KeyWord:palmitic acid lipidomics HPLC-MS cell cycle |
| FundProject:国家重点研发计划（2017YFD0400200） |
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| Abstract: |
| Lipidomics analysis of lipids in palmitic acid (PA)-treated human-derived hepatocytes was performed to explore the effect of PA on hepatocyte lipid metabolism. HPLC-MS-based lipidomics technique was used to identify and quantify the endogenous lipid metabolites altered after PA administration, and cell cycle analysis was performed by flow cytometer. The results showed that 1 000 lipid metabolites subdivided in to 22 lipid classes were identified by lipidomics analysis and 628 lipid species levels showed significant difference according to the orthogonal partial least squares discriminant analysis (OPLS-DA). Among them, lysophosphatidylcholine (LPC), phosphatidylinositol (PI), ceramide (Cer), hexose1ceramide (Hex1Cer), sphingosine (SPH), diglyceride (DG), triglyceride (TG), acetylcarnitine (AcCa) and N-acyl ethanolamine (AEA) were significantly higher in the PA-treated group, and sphingomyelin (SM), hexose2ceramide (Hex2Cer) and cholesteryl ester (ChE) significantly decreased. Compared with the control group, the proportion of cells in G0/G1 phase decreased and those in S and G2/M phase increased in the PA-treated group.In conclusion, PA significantly altered the lipid metabolic profile of hepatocytes and induced cellular G2/M phase arrest. |
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