Selecting flaxseed from different producing areas in Qinghai province as raw materials, flaxseed oil was extracted by Soxhlet extraction method, and the fatty acid composition of flaxseed oil was analyzed by gas chromatography, then the fatty acid standard fingerprint was constructed using the similarity evaluation system of chromatographic fingerprint of traditional Chinese medicine. The adulteration models of 10%, 20%, 30%, 40% and 50% of soybean oil, corn oil, peanut oil, rapeseed oil, sunflower seed oil and sesame oil mixed in flaxseed oil were identified by the constructed fingerprint. The results showed that the fingerprint constructed met the technical requirments for fingerprinting studies and could represent the overall properties of Qinghai flaxseed oil to a certain extent. The fingerprint could identify adulteration amount of equal to and above 20% soybean oil and sunflower seed oil, equal to and above 10% corn oil, peanut oil and rapeseed oil. The identification effect of sesame oil adulteration was not good, and the identification could only be performed when the adulteration amount was above 30%. |