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Optimization of extraction process of polysaccharides from Camelina sativa (L.) Crantz seeds cake with response surface methodology and its antioxidant activity |
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DOI: |
KeyWord:Camelina sativa (L.) Crantz seeds polysaccharides extraction antioxidant activity |
FundProject:河南省科学院重大科研项目聚焦专项(190113004);河南省科学院基础科学研究项目(200613114) |
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Abstract: |
Taking Camelina sativa (L.) Crantz seeds cake as the raw material, the extraction technology of polysaccharides by constant temperature stirring was optimized by response surface methodology based on single factor experiment. The in vitro antioxidant activities of the extract were measured. The results showed that the optimal extraction conditions were obtained as follows∶ extraction temperature 90 ℃, solid-liquid ratio 1∶ 18, extraction time 85 min and stirring speed 200 r/min. Under the optimal conditions, the polysaccharides yield was 1.71% when being extracted twice. The crude polysaccharide extract exhibited certain scavenging effects on DPPH·and ·OH, but the effect on latter was stronger than the former. The results could provide theoretical basis for the extraction and application of polysaccharides of Camelina sativa (L.) Crantz seeds. |
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