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| Determination of squalene in vegetable oil by gas chromatography-mass spectrometry with rapid saponification-neutral alumina chromatographic purification |
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| DOI： |
| KeyWord:vegetable oil squalene GC GC-MS |
| FundProject:国家重点研发计划项目（2019YFD1002400，2019YFD1002403）；陕西省功能油脂科技创新团队项目(2018-TD-020)；陕西省技术创新引导专项——区域创新能力引导计划项目（2020QFY08-03）；陕西省林业科学院科技创新计划专项重点项目（SXLK2020-0213） |
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| Abstract: |
| In order to determine squalene in vegetable oil quickly and accurately, through the optimization of sample pretreatment process and exploration of mass spectrometry analysis conditions, the method for determination of squalene in vegetable oil was established with a rapid saponification-neutral alumina chromatographic purification and gas chromatography-mass spectrometry(GC-MS). The results showed that the peaks of squalene and other impurities in vegetable oil samples were well separated by GC-MS with internal standard method in the selective ion monitoring mode. The correlation coefficient of the regression equation of the standard curve in the mass concentration range of 0-194.4 μg/mL was 0.998 6, which indicated that the linear relationship was good. The coefficient of variation of the method establised was 1.3%-2.6%, and the spiked recovery rate was 95.0%-96.7%. Compared with LS/T 6120-2017, the method established had the advantages of rapid and simple sample pretreatment, good precision, and high sensitivity, which could greatly improve the detection efficiency of squalene. This method established was used to test the squalene content in 10 vegetable oils, and squalene was detected in all of these vegetable oils, with olive oil containing the highest amount of squalene at approximately 6 000 mg/kg. |
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