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| 快速皂化-中性氧化铝层析净化气相色谱- 质谱法测定植物油中的角鲨烯 |
| Determination of squalene in vegetable oil by gas chromatography-mass spectrometry with rapid saponification-neutral alumina chromatographic purification |
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| DOI： |
| 中文关键词: 植物油 角鲨烯 气相色谱法 气相色谱-质谱法 |
| 英文关键词:vegetable oil squalene GC GC-MS |
| 基金项目:国家重点研发计划项目（2019YFD1002400，2019YFD1002403）；陕西省功能油脂科技创新团队项目(2018-TD-020)；陕西省技术创新引导专项——区域创新能力引导计划项目（2020QFY08-03）；陕西省林业科学院科技创新计划专项重点项目（SXLK2020-0213） |
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| 中文摘要: |
| 为了快速准确地测定植物油中的角鲨烯，通过对样品前处理过程优化和质谱分析条件的摸索，建立了快速皂化-中性氧化铝层析净化气相色谱-质谱法（GC-MS）测定植物油中角鲨烯的方法。结果表明：GC-MS选择离子监测模式内标法定量下，植物油样品中角鲨烯与其他干扰物质得到了良好的分离；在0～194.4 μg/mL质量浓度范围内标准曲线回归方程的相关系数为0.998 6，线性关系良好；方法的变异系数为1.3%～2.6%，加标回收率为95.0%～96.7%；与LS/T 6120—2017相比，所建立的方法具有样品前处理快速简单、精密度好、灵敏度高等优点, 大大提高了角鲨烯的检测效率；采用该方法对10种植物油中角鲨烯含量进行检测，10种植物油中均有检出，其中橄榄油中角鲨烯含量最高，约为6 000 mg/kg。 |
| 英文摘要: |
| In order to determine squalene in vegetable oil quickly and accurately, through the optimization of sample pretreatment process and exploration of mass spectrometry analysis conditions, the method for determination of squalene in vegetable oil was established with a rapid saponification-neutral alumina chromatographic purification and gas chromatography-mass spectrometry(GC-MS). The results showed that the peaks of squalene and other impurities in vegetable oil samples were well separated by GC-MS with internal standard method in the selective ion monitoring mode. The correlation coefficient of the regression equation of the standard curve in the mass concentration range of 0-194.4 μg/mL was 0.998 6, which indicated that the linear relationship was good. The coefficient of variation of the method establised was 1.3%-2.6%, and the spiked recovery rate was 95.0%-96.7%. Compared with LS/T 6120-2017, the method established had the advantages of rapid and simple sample pretreatment, good precision, and high sensitivity, which could greatly improve the detection efficiency of squalene. This method established was used to test the squalene content in 10 vegetable oils, and squalene was detected in all of these vegetable oils, with olive oil containing the highest amount of squalene at approximately 6 000 mg/kg. |
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