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| Separation of antioxidant activity components from black cumin seed oil by deep eutectic solvents |
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| KeyWord:deep eutectic solvents black cumin seed oil antioxidant activity components |
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| Abstract: |
| Thirteen deep eutectic solvents (DES) were prepared for separation of antioxidant activity components from black cumin seed oil. The extraction efficiency of different DES was studied based on the DPPH free radical scavenging capability of DES extracts. The antioxidant components in the DES extract with the highest DPPH free radical scavenging capability were further separated by rapid preparation chromatography, and characterized by UPLC-Q-TOF MS. The results showed that the DES-9(betaine-levulinic acid-water, molar ratio 1∶ 3∶ 2) extract had the highest antioxidant activity, and the DPPH free radical scavenging rate of DES-9 extract was 26 times that of traditional solvent (70% methanol) extract at the same raw material concentration (378 mg/mL). The antioxidant components in the DES-9 extract were mainly 5-(3,5-dimethoxy-4-methylphenyl) pentanoic acid, sugetriol, cinnamic acid, odoratol, 2-O-2-phenylethyl-β-D-glucopyranoside, thymoquinone, crocetin and carnosic acid. |
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