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| 高效液相色谱-荧光法测定食用油中 苯并（a）芘残留量 |
| Determination of benzo（a）pyrene residues in edible oil by HPLC-fluorescence method |
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| DOI： |
| 中文关键词: 苯并（a）芘 残留 食用油 高效液相色谱 荧光检测器 |
| 英文关键词:benzopyrene residual edible oil HPLC fluorescence detector |
| 基金项目:国家重点研发计划项目（2018YFC1602101）；四川省科技计划重点研发项目（2019YFN0172）；四川省科技成果转移转化项目（20ZHSF0154）；四川省应用基础研究计划项目（19YYJC1846）；成都市科技局技术创新研发项目（2019-YF05-02192-SN）；农业农村部杂粮加工重点实验室开放课题资助 |
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| 中文摘要: |
| 建立了高效液相色谱-荧光法测定食用油中苯并（a）芘残留量的方法。样品经正己烷提取、苯并（a）芘分子印迹柱净化后，进高效液相色谱仪，在RP-C18保护柱Welch C18 色谱柱（250 mm×4.6 mm×5 μm）、88%乙腈-水溶液为流动相、进样量10 μL条件下，用荧光检测器（激发波长384 nm，发射波长406 nm）测定食用油中苯并（a）芘含量。结果表明：该方法的苯并（a）芘线性范围为1～10 ng/mL，相关系数为0.999 91，检出限为0.2 μg/kg，平均加标回收率为94.9%。采用该方法对7种市售食用油中苯并（a）芘进行测定，结果发现，玉米胚芽油、鱼油中未检出苯并（a）芘，黑芝麻油中苯并（a）芘含量为2.780 μg/kg，胡麻籽油中苯并（a）芘含量为0.863 μg/kg，葵花仁油中苯并（a）芘含量为0.238 μg/kg，大蒜油中苯并（a）芘含量为0.410 μg/kg，五味子油中苯并（a）芘含量为0.132 μg/kg，均低于国家规定限量（10 μg/kg）。与国标法相比，该方法操作简便，节约时间，且灵敏度高、回收率稳定，适用于多种食用油中苯并（a）芘残留量的快速检测分析。 |
| 英文摘要: |
| A HPLC - fluorescence method for determination of benzo(a)pyrene(BaP) residues in edible oil was established. The sample was extracted by n-hexane, and purified by BaP molecular imprinted column, then injected into HPLC, under the conditions of RP-C18 as protective column, Welch C18 (250 mm×4.6 mm×5 μm) as chromatographic column, 88% acetonitrile-aqueous as mobile phase, and sampling volume 10 μL, the residue of BaP in edible oil was determined by fluorescence detector (excitation wavelength 384 nm, emission wavelength 406 nm). The results showed that the linear range of BaP was 1-10 ng/mL, the correlation coefficient was 0999 91, the detection limit was 0.2 μg/kg, and the average standard recovery rate was 94.9%. The BaP contents of seven vegetable oils on sale were determined by the method, and it was showed that no BaP was detected in corn germ oil and fish oil.The contents of BaP in black sesame oil, flaxseed oil, sunflower seed kernel oil, garlic oil, and Schisandrae fructus oil was 2.780,0.863,0.238,0.410,0.132 μg/kg respectively, which were lower than the national limit of 10 μg/kg.Compared with the national standard method, this method was simple, time-saving, sensitive and stable, and were suitable for the rapid detection and analysis of BaP residues in various edible oils. |
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