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| HPLC测定亚麻荠种子中芦丁及指纹图谱研究 |
| Determination of rutin in camelina seed by HPLC and its fingerprint |
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| DOI： |
| 中文关键词: 亚麻荠种子 芦丁 HPLC 指纹图谱 |
| 英文关键词:camelina seed rutin HPLC fingerprint |
| 基金项目:河南省科学院国内外访学项目（210813005）；河南省科学院重大聚焦项目（190113004) |
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| 中文摘要: |
| 为更好地评价亚麻荠种子的质量，采用HPLC测定12批亚麻荠种子的芦丁含量，并建立了亚麻荠种子的HPLC指纹图谱，通过TCMYS指纹图谱软件对不同产地亚麻荠种子指纹图谱进行相似度分析。结果表明，HPLC测定芦丁的标准曲线回归方程线性关系良好（r=0.999）,平均加标回收率为99.37%，12批亚麻荠种子指纹图谱相似度较高，标定16个共有峰。HPLC指纹图谱方法稳定性及重复性良好，可为亚麻荠种子的质量评价及质量控制提供依据。 |
| 英文摘要: |
| In order to better evaluate the quality of camelina seeds, the rutin contents of 12 batches of camelina seeds were determined by HPLC, and its HPLC fingerprints were established. The similarity analysis was performed on fingerprints of camelina seeds from different producing areas by TCMYS fingerprint software. The results showed that the regression equation of the standard curve for the determination of rutin by HPLC had a good linear relationship (r=0.999), and the average spiked recovery was 99.37%. The fingerprints of 12 batches of camelina seeds had high similarity, and sixteen common peaks were found. The HPLC fingerprint methods had good stability and reproducibility, which could provide a basis for quality evaluation and quality control of camelina seeds. |
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