| The content of unsaponifiable matter in rubber seed oil was measured according to national standard GB/T 5535.2-2008. Then the components of unsaponifiable matter in rubber seed oil were further analyzed by gas chromatography-mass spectrometry (GC-MS). The antioxidant activities of rubber seed oil and its unsaponifiable components were evaluated by DPPH· and ABTS· methods. The results showed that the unsaponifiable matter content in rubber seed oil was (1.44±0.03)%, there were 13 kinds of unsaponifiable matter in rubber seed oil, mainly including γ-sitosterol (40.24%), stigmasterol (1720%), 3, 4-dihydro-2, 2, 5, 7, 8-pentamethyl-2H-1-benzopyrene-6-phenol (9.53%), 4-cholesterin-3-ketone (6.64%), squalene (6.14%), campesterol (437%), D-γ-(P)-tocotrienol (327%) and etc. Rubber seed oil and its unsaponifiable matter had good antioxidant activity, and their IC50 values of DPPH· scavenging rate were 6.979 mg/mL and 3.819 mg/mL, their IC50 values of ABTS· scavenging rate were 16.979 mg/mL and 4.356 mg/mL, respectively. |
