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| Biomolecules mobility on milk fat globule membrane using fluorescence recovery after photobleaching (FRAP) |
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| DOI： |
| KeyWord:human milk fat milk fat globule membrane polar lipids fluorescence recovery after photobleaching mobility |
| FundProject:国家自然科学基金资助项目(31701558) |
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| Abstract: |
| In order to promote the accurate evaluation of milk products, a high-resolution confocal laser microscope (CLSM) combined with multi-fluorescence probe technology was used to observe the microstructure of different mammalian milk, including human milk, cow milk, and goat milk, and fluorescence recovery after photobleaching (FRAP) was used to quantitatively detect the biomolecules mobility on the milk fat globule membrane in different mammalian milk.The results showed that the microstructures of the three kinds of milk were basically the same,and crescents areas appeared in the CLSM diagram of the three kinds of milk were proved to be the local phospholipids enrichment areas. The mobile fraction of triacylglycerol and water soluble proteins in milk fat globules was relatively high, the mobile fraction of polar lipids and phosphatidylcholine in different milk was relatively close,while the sphingomyel showed the lowest mobile fraction. The mobility of triglycerides in cow milk was slower than that of goat milk and human milk, and the mobility of polar lipids in human milk was the slowest. FRAP could intuitively characterize the biomolecules mobility on the milk fat globule membrane, and could provide a new method for the study of the biochemical properties of the membrane interface in the emulsion. |
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