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| 超高效合相色谱（UPC2）技术快速测定植物油中的维生素A和维生素E |
| Rapid determination of vitamin A and vitamin E in vegetable oil based on ultra high performance convergence chromatography(UPC2 |
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| DOI： |
| 中文关键词: 超高效合相色谱法 植物油 维生素A 维生素E |
| 英文关键词:ultra high performance convergence chromatography vegetable oil vitamin A vitamin E |
| 基金项目: |
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| 中文摘要: |
| 为了使植物油中脂溶性维生素E异构体得到更好的分离，建立一种超高效合相色谱（UPC2）快速分离和测定植物油中维生素A和维生素E的方法。样品经异丙醇溶解，过0.22 μm有机相滤膜后直接进样分析。分析条件为HSS C18 SB色谱柱（3.0 mm×100 mm,1.8 μm），CO2和0.2%甲酸甲醇溶液为流动相，梯度洗脱，流速1.0 mL/min，柱温50 ℃，检测波长290 nm。结果表明：维生素A和维生素E在测定范围内呈现良好的线性关系，相关系数均在0.999 5及以上，样品回收率为82.00%～106.82%，RSD在0.94%～3.57%之间，日内精密度与日间精密度的RSD分别为1.94%～3.51%与3.74%～5.13%，并采用已建立的方法对26份植物油样品中的维生素A和维生素E含量进行测定，表明植物油中维生素E含量最高，其中以α-生育酚和γ-生育酚为主，β-生育酚含量较低。该方法简单高效、灵敏度高、重复性好，可用于植物油中脂溶性维生素A和维生素E的分离检测。 |
| 英文摘要: |
| In order to better separate the fat-soluble vitamin E isomers in vegetable oils, a method for rapid separation and determination of vitamin A and vitamin E in vegetable oil by ultra high performance convergence chromatography was established. The sample was dissolved in isopropanol and passed through a 0.22 μm organic phase filter membrane, and then directly injected for analysis. The analysis conditions were as follows: HSS C18 SB chromatographic column (3.0 mm×100 mm, 1.8 μm), with CO2 and 0.2% formic acid methanol solution as mobile phase, gradient elution, flow rate 1.0 mL/min, column temperature 50 ℃, detection wavelength 290 nm. The results showed that vitamin A and vitamin E showed a good linear relationships in the determination range, the correlation coefficients were not less than 0.999 5, the sample recovery was 82.00%-106.82%, the RSD was 0.94%-3.57%, and the RSD of intraday precision and daytime precision were 1.94%-3.51% and 3.74%-5.13%, respectively. The contents of vitamin A and vitamin E in 26 vegetable oil samples were determined by the established method. The vitamin E content was the highest, in which α-tocopherol and γ-tocopherol was the main tocopherol, while β-tocopherol conternt was lower. The method is simple, efficient, sensitive and reproducible. The method can be used for the separation and determination of vitamin A and vitamin E in vegetable oil. |
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