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| Optimization of extraction conditions of Aspongopus chinensis Dallas oil and its anti-breast cancer activity in vitro |
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| DOI： |
| KeyWord:Aspongopus chinensis Dallas Aspongopus chinensis Dallas oil Soxhlet extraction method anti-breast cancer activity |
| FundProject:贵州省科技支撑计划项目（黔科合支撑\[2021\]一般180） |
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| Abstract: |
| In order to promote the development of the oil from Aspongopus chinensis Dallas, single factor experiment and orthogonal experiment were used to optimize the extraction conditions of A. chinensis Dallas oil by Soxhlet extraction method. Meanwhile, CCK-8 method was used to detect the effects of the extracted oil on the proliferation of mouse breast cancer cells 4T1 and human breast cancer cells HCC1937. The results showed that the optimal extraction conditions of A. chinensis Dallas oil were obtained as follows: liquid-solid ratio 6∶ 1, extraction temperature 90 ℃, extraction time 3 h. Under the optimal conditions, the extraction rate of oil was (44.270±1.679)%. The A. chinensis Dallas oil with the mass concentration of 4, 8, 12, 16 mg/mL treating for 24 h could inhibit the proliferation of two types of breast cancer cells, and the effect on human breast cancer cells HCC1937 was better than that on mouse breast cancer cells 4T1. Moreover, the morphology and quantity of the two types of cells were significantly changed by A. chinensis Dallas oil treatment. In summary, A. chinensis Dallas oil obtained has a significant inhibitory effect on breast cancer cells 4T1 and HCC1937 in vitro. |
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