|
| Characterization and application of carbon dots from rice bran protein |
| |
| DOI： |
| KeyWord:rice bran protein carbon dots fluorescence performance Fe3+ sensing cell imaging |
| FundProject:生物医学分析化学教育部重点实验室（武汉大学）开放课题(ACBM2019003)；湖北省科技重大专项（2019ABA105）；湖北省粮食科技创新项目 |
|
| Hits: 1364 |
| Download times: 999 |
| Abstract: |
| The carbon dots (CDs) were prepared by hydrothermal method using rice bran protein(RBP) as the carbon source. The optical properties, morphology and surface functional groups of the prepared rice bran protein carbon dots (RBP-CDs) were characterized by UV-vis absorption spectrum, fluorescence spectrum, high-resolution transmission electron microscope (TEM) and Fourier transform infrared spectroscopy (FTIR), the stability of RBP-CDs in different environments and their application in sensing, cell imaging and fluorescent staining were investigated. The results showed that RBP-CDs had a UV absorption peak at 286 nm, and its fluorescence emission wavelength was excitation-dependent. RBP-CDs were approximately spherical with good dispersibility and the uniform size, mainly concentrated in 2-4 nm. The surface of RBP-CDs was rich in hydroxyl and carboxyl groups, so the hydrophilic property was better. In the pH 4-10 or high-salt environment, RBP-CDs had good fluorescence performance; under the long-term irradiation of 365 nm ultraviolet lamp, the fluorescence intensity did not change much, showing good optical stability; but the fluorescence intensity was different under different chemical environments, which was mainly related to the defect state of the RBP-CDs surface. The fluorescence sensing experiments showed that the RBP-CDs could be used as a “Turn-Off” fluorescence sensor for Fe3+, with a detection limit of 15.2 nmol/L,and it had lower cytotoxicity and could be used for staining and imaging of HepG-2 cells. RBP-CDs could be used as a new type of fluorescent labeling material for the cell labeling and cell imaging. |
| View full text View/Add Comment Download reader |
| Close |
|
|
|
