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| Extraction of Moringa oleifera seed antimicrobial peptides by E.coli cell membrane stationary phase |
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| DOI： |
| KeyWord:Escherichia coli E.coli cell membrane stationary phase Moringa oleifera seed antimicrobial peptide extraction antibacterial rate |
| FundProject:国家自然科学基金地区项目（31960462）；云南省基础研究计划面上项目（2019FB052）；云南省农业联合专项（2018FG001-040）；云南省教育厅科学研究基金项目（2019J0114） |
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| Abstract: |
| E. coli cell membrane stationary phase (ECMSP) was prepared, and its extraction effect of Moringa oleifera seed antimicrobial peptides was studied. The effects of ultrasonic time and ultrasonic power on the wall breaking effect of E. coli were studied. The adsorption characteristics of E. coli cell membrane and macroporous silica gel, and the adsorption effect of ECMSP on the Moringa oleifera seed antimicrobial peptides were analyzed. The change of antibacterial activity and the reverse-high performance liquid chromatography (RP-HPLC) difference peaks before and after extraction were clarified. The results showed that when the ultrasonic power was 500 W and the ultrasonic time was 30 min, the wall breaking effect was good. The saturated adsorption value （Csmax）of the macroporous silica gel to the cell membrane of E. coli was 9.98 μg/mg, and the adsorption constant (K*) was 171.02 μg/mL. The inhibitory effects of antimicrobial peptides on Escherichia coli and Staphylococcus aureus were significantly different, and RP-HPLC analysis showed three different peaks before and after extraction. The research could provide theoretical basis and application references for the subsequent development and utilization of Moringa oleifera seed antimicrobial peptides. |
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