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| Optimization of fermentation conditions of Neurospora crassa in the presence of oleic acid for promoting lycopene production |
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| KeyWord:lycopene Neurospora crassa oleic acid mevalonate pathway |
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| Abstract: |
| The lycopene production through microbial fermentation method is an effective alternative strategy for direct extraction and chemical synthesis. With oleic acid as exogenous fatty acid, the effect of oleic acid on lycopene production in Neurospora crassa was studied, and the response surface methodology was used to determine the optimal fermentation conditions, and the levels of enzymes associated with lycopene synthesis were further studied in the presence or absence of oleic acid. The results showed that adding oleic acid to the medium significantly increased the lycopene yield in N.crassa, and the optimal fermentation conditions were obtained as follows: oleic acid mass concentration 0.90 g/L, shaking rate 100 r/min, shaking culture temperature 34 ℃ and static culture temperature 30 ℃. Under these conditions, lycopene yield was 4.21 mg/L, which was 2.77 times that of the control group. Moreover, oleic acid added in the medium could not only increase the biomass of N. crassa, but also significantly increase the acetyl-CoA content compared with the control group. Meanwhile, the levels of enzymes associated with lycopene synthesis (HMG-CoA reductase, HMG-CoA synthase and geranylgeranyl pyrophosphate synthase) also significantly increased. These results suggested that more available acetyl-CoA could enter the mevalonate pathway and further promote the synthesis of lycopene under the action of the above enzymes by adding oleic acid. |
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