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| 油酸促进粗糙脉孢菌发酵高产番茄红素的条件优化 |
| Optimization of fermentation conditions of Neurospora crassa in the presence of oleic acid for promoting lycopene production |
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| DOI： |
| 中文关键词: 番茄红素 粗糙脉孢菌 油酸 甲羟戊酸途径 |
| 英文关键词:lycopene Neurospora crassa oleic acid mevalonate pathway |
| 基金项目: |
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| 中文摘要: |
| 微生物发酵法生产番茄红素是直接提取和化学合成的有效替代策略。以油酸为外源脂肪酸，探究其对粗糙脉孢菌发酵产番茄红素的影响，通过响应面法优化确定最佳培养条件，并进一步探究油酸对番茄红素合成相关酶的影响。结果表明：在含油酸的培养基中，粗糙脉孢菌的番茄红素产量显著增加；在油酸质量浓度0.90 g/L、振荡速率100 r/min、振荡培养温度34 ℃、静置培养温度30 ℃条件下，番茄红素产量达到4.21 mg/L，是空白对照组的2.77倍。培养基中油酸的添加不仅促进了粗糙脉孢菌生物量的积累，菌体内乙酰辅酶A的水平也显著增加；同时，与番茄红素合成相关酶（HMG-CoA合成酶、HMG-CoA还原酶、香叶基香叶基焦磷酸合酶）的水平亦显著升高。结果表明油酸的添加可供利用更多的乙酰辅酶A进入甲羟戊酸途径，并进一步在上述酶的诱导作用下促进番茄红素的合成。 |
| 英文摘要: |
| The lycopene production through microbial fermentation method is an effective alternative strategy for direct extraction and chemical synthesis. With oleic acid as exogenous fatty acid, the effect of oleic acid on lycopene production in Neurospora crassa was studied, and the response surface methodology was used to determine the optimal fermentation conditions, and the levels of enzymes associated with lycopene synthesis were further studied in the presence or absence of oleic acid. The results showed that adding oleic acid to the medium significantly increased the lycopene yield in N.crassa, and the optimal fermentation conditions were obtained as follows: oleic acid mass concentration 0.90 g/L, shaking rate 100 r/min, shaking culture temperature 34 ℃ and static culture temperature 30 ℃. Under these conditions, lycopene yield was 4.21 mg/L, which was 2.77 times that of the control group. Moreover, oleic acid added in the medium could not only increase the biomass of N. crassa, but also significantly increase the acetyl-CoA content compared with the control group. Meanwhile, the levels of enzymes associated with lycopene synthesis (HMG-CoA reductase, HMG-CoA synthase and geranylgeranyl pyrophosphate synthase) also significantly increased. These results suggested that more available acetyl-CoA could enter the mevalonate pathway and further promote the synthesis of lycopene under the action of the above enzymes by adding oleic acid. |
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