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| 酶法联合超声波提取亚麻籽胶 |
| Extraction of flaxseed gum by enzymatic method combined with ultrasound |
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| DOI： |
| 中文关键词: 亚麻籽胶 亚麻籽粕 酶法 超声波 |
| 英文关键词:flaxseed gum flaxseed meal enzymatic method ultrasound |
| 基金项目:河北省科技厅项目（18227132D）：河北省教育厅项目（ZD2017205） |
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| 中文摘要: |
| 以脱脂亚麻籽粕为原料，采用酶法联合超声波提取其中的亚麻籽胶。以亚麻籽胶得率为指标，筛选提取亚麻籽胶的最适酶制剂，在单因素试验基础上，采用正交试验对酶添加量、提取温度、提取时间、料液比、超声功率进行优化，并对提取的亚麻籽胶中单糖组成及含量进行测定，对其结构进行红外光谱表征。结果表明：最佳酶制剂为果胶酶；亚麻籽胶最佳提取工艺条件为酶添加量125%、提取温度40 ℃、提取时间30 min、料液比（脱脂亚麻籽粕与水的质量体积比）1∶ 30、超声功率400 W，在此条件下亚麻籽胶得率为33.41%；亚麻籽胶中单糖的组成及含量分别为甘露糖097%、鼠李糖14.05%、葡萄糖醛酸0.16%、半乳糖醛酸22.17%、葡萄糖3.76%、半乳糖1744%、木糖23.53%、阿拉伯糖10.30%、岩藻糖7.62%。 |
| 英文摘要: |
| Defatted flaxseed meal was used as raw material, and flaxseed gum was extracted from it by enzymatic method combined with ultrasound. With the yield of flaxseed gum as indicator, the optimal enzyme for extracting flaxseed gum was selected. The enzyme dosage, extraction temperature, extraction time, material-liquid ratio and ultrasonic power were optimized by single factor experiment and orthogonal experiment. The monosaccharide composition and content of flaxseed gum were determined, and its structure was characterized by FT-IR. The results showed that the best enzyme was pectinase. The optimal extraction process of flaxseed gum were obtained as follows: enzyme dosage 1.25%, extraction temperature 40 ℃, extraction temperature 30 min, ratio of defatted flaxseed meal mass to water volume 1∶ 30, ultrasonic power 400 W. Under the optimal conditions, the yield of flaxseed gum was 3341%.The monosaccharide composition and content of flaxseed gum were 0.97% of mannose, 1405% of rhamnose, 0.16% of glucuronic acid, 2217% of galacturonic acid, 3.76% of glucose, 1744% of galactose, 23.53% of xylose, 1030% of arabinose and 7.62% of fucose. |
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