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| Optimization of simultaneous extraction of oil, protein and gum from flaxseed by enzyme-assisted three phase partitioning |
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| DOI： |
| KeyWord:flaxseed oil flaxseed protein flaxseed gum three phase partitioning aqueous enzymatic method simultaneous extraction |
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| Abstract: |
| Enzyme-assisted three phase partitioning (EATPP) was used to simultaneously extract oil, protein and gum from flaxseed, and the extraction rate of flaxseed oil was used as an indicator to optimize the process conditions of EATPP by single factor experiment and response surface methodology. The results showed that the optimal EATPP process conditions were obtained as follows: enzymatic hydrolysis time 2 h, enzyme dosage 433 U/g, tert-butanol dosage 3.9 mL/g, ammonium sulfate dosage 2.1 g/g, three phase extraction temperature 45 ℃, three phase extraction time 4 h. Under the optimal process conditions, the extraction rate of the flaxseed oil from three varieties reached 84.47%-8903%, the extraction rate of flaxseed protein reached 50.58%-55.69%, and the extraction rate of flaxseed gum reached 31.62%-35.61%. Using EATPP can not only simultaneously extract oil, protein and gum from flaxseed, but also can reduce cost and improve the utilization rate of flaxseed. |
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